Catalog number

R1001.2

Description

This all-in-one RNA Synthesis Kit with directly cap1 analog is designed to produce large amounts of cap1 capped N1-methyl-pseudoUTP RNA via in vitro transcription with T7 RNA Polymerase. The resulting RNA can subsequently be used for a variety of applications such as microinjection, transfection or in vitro translation experiments. The kit contains sufficient reagents for 20 reactions of 50μleach. A typical reaction yields about 40-160μg RNA after 2h incubation (1μg T7 control template, 1.4 kb RNA transcript).Yields may however vary depending on the template (promotor design, sequence length, secondary structure formation).